NMR Restraints Grid |
Result table
image | mrblock_id | pdb_id | cing | stage | position | type |
6536 | 1j6t | cing | 1-original | 13 | comment |
(2) Ca-Ha and N-C' dipolar couplings. Da(NH) = -9.4 Hz, rhombicity = 0.58 measured in 4% PEG/hexanol using 15N/13C-HPr/unlabeled-IIAmtl and unlabeled-HPR/15N/13C-IIAmtl with unlabeled component in large excess to ensure that labeled protein was completely bound. Note that small unavoidable variations in the concentration of PEG/hexanol among different samples introduces small variations in Da(NH) (the axial component of the alignment tensor). The dipolar couplings are appropriately normalized to reflect this. Also all dipolar couplings are normalized to Da(NH).
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