NMR Restraints Grid

Result table
 (Save to zip file containing files for each block)

image	mrblock_id	pdb_id	bmrb_id	cing	stage	program	type
	610365	5t1o	30159	cing	1-original	MR format	comment

*HEADER    TRANSFERASE                             19-AUG-16   5T1O              
*TITLE     SOLUTION-STATE NMR AND SAXS STRUCTURAL ENSEMBLE OF NPR (1-85) IN      
*TITLE    2 COMPLEX WITH EIN-NTR (170-424)                                       
*COMPND    MOL_ID: 1;                                                            
*COMPND   2 MOLECULE: PHOSPHOCARRIER PROTEIN NPR;                                
*COMPND   3 CHAIN: A;                                                            
*COMPND   4 SYNONYM: NITROGEN-RELATED HPR;                                       
*COMPND   5 EC: 2.7.11.-;                                                        
*COMPND   6 ENGINEERED: YES;                                                     
*COMPND   7 MOL_ID: 2;                                                           
*COMPND   8 MOLECULE: PHOSPHOENOLPYRUVATE-PROTEIN PHOSPHOTRANSFERASE PTSP;       
*COMPND   9 CHAIN: B;                                                            
*COMPND  10 SYNONYM: ENZYME I-NTR,PHOSPHOTRANSFERASE SYSTEM,ENZYME I;            
*COMPND  11 EC: 2.7.3.9;                                                         
*COMPND  12 ENGINEERED: YES;                                                     
*COMPND  13 MUTATION: YES;                                                       
*COMPND  14 OTHER_DETAILS: THIS MOLECULE WAS MUTATED AT POSITION 356 FROM        
*COMPND  15 HISTIDINE TO GLUTAMINE. IN E. COLI, WHEN THE NATIVE HISTIDINE IS     
*COMPND  16 PRESENT, THE SAMPLE CAN BE PHOSPHORYLATED. IT WAS MUTATED TO PROVIDE 
*COMPND  17 A HOMOGENEOUS SAMPLE. ADDITIONALLY, IT COMPRISES RESIDUES 170-424 OF 
*COMPND  18 THE LARGER ENZYME I-NTR MOLECULE. RESIDUE 169 IS MUTATED TO GLYCINE  
*COMPND  19 DUE TO TEV PROTEASE CLEAVAGE.                                        
*SOURCE    MOL_ID: 1;                                                            
*SOURCE   2 ORGANISM_SCIENTIFIC: ESCHERICHIA COLI O157:H7;                       
*SOURCE   3 ORGANISM_TAXID: 83334;                                               
*SOURCE   4 GENE: PTSO, Z4569, ECS4085;                                          
*SOURCE   5 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
*SOURCE   6 EXPRESSION_SYSTEM_TAXID: 562;                                        
*SOURCE   7 EXPRESSION_SYSTEM_STRAIN: ER2566;                                    
*SOURCE   8 EXPRESSION_SYSTEM_PLASMID: PLASMID;                                  
*SOURCE   9 MOL_ID: 2;                                                           
*SOURCE  10 ORGANISM_SCIENTIFIC: ESCHERICHIA COLI (STRAIN K12);                  
*SOURCE  11 ORGANISM_TAXID: 83333;                                               
*SOURCE  12 STRAIN: K12;                                                         
*SOURCE  13 GENE: PTSP, YGDF, YGDO, B2829, JW2797;                               
*SOURCE  14 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
*SOURCE  15 EXPRESSION_SYSTEM_TAXID: 562;                                        
*SOURCE  16 EXPRESSION_SYSTEM_STRAIN: ER2566;                                    
*SOURCE  17 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
*SOURCE  18 EXPRESSION_SYSTEM_PLASMID: PET28A                                    
*KEYWDS    PTSNTR, PHOSPHOTRANSFER, BACTERIAL, COMPLEX, TRANSFERASE              
*EXPDTA    SOLUTION NMR                                                          
*NUMMDL    20                                                                    
*AUTHOR    M.STRICKLAND,A.M.STANLEY,G.WANG,C.D.SCHWIETERS,S.BUCHANAN,            
*AUTHOR   2 A.PETERKOFSKY,N.TJANDRA                                              
*REVDAT   1   16-NOV-16 5T1O    0                                                

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