## NMR Restraints Grid |

Result table

image |
mrblock_id |
pdb_id |
cing |
stage |
position |
program |
type |

520493 | 108d | cing | 1-original | 2 | MR format | comment |

REMARK REMARK H2* 4 is a pseudo atom at the geometric center of REMARK the thymidine residue 4 1H2' and 2H2' protons. REMARK REMARK H2* 12 is a pseudo atom at the geometric center of REMARK the thymidine residue 12 1H2' and 2H2' protons. REMARK REMARK restraints generated by using the randmardi REMARK procedure of the program MARDIGRAS. NOESY data for both upper REMARK and lower sides of the diagonal for five mixing times (25 ms, 50 REMARK ms, 100 ms, 150 ms, 200 ms) corrected for T1 relaxation was REMARK used. Randmardi was implemented using 30 cycles for each of the REMARK ten data sets where the random noise level was set to between REMARK one and five times the integrated intensity of the smallest REMARK cross peak in the spectrum. The results were combined using the REMARK program AVGBNDS (Liu, H., James, T.L. University of California, REMARK San Francisco), and the lower and upper bounds expressed as the REMARK average calculated interproton distance +/- one standard REMARK deviation for that proton pair. The upper bounds were furthur REMARK modified by adding an extra 0.2 Angsroms to them. The bounds for REMARK symmetry related restraints were averaged. The correlation time REMARK used in the relaxation matrix calculations was 3.75ns. REMARK REMARK REMARK LOWER UPPER REMARK ATOM 1 ATOM 2 BOUND BOUND

Please acknowledge these references in publications where the data from this site have been utilized.

Contact the webmaster for help, if required. Tuesday, July 17, 2018 2:41:01 AM CDT (wattos1)