NMR Restraints Grid

Result table
 (Save to zip file containing files for each block)

image	mrblock_id	pdb_id	bmrb_id	cing	stage	position	type
	3936	1egf	2201	cing	1-original	2	comment

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The pseudo-atom (QA, QB, QG, QE, QQG, QQD, CG1, and CZ4) names 
are those described in Wuethrich, et al.[J. Mol. Biol.169: 949(1983)]. 
The side-chain amide protons (HDE, HDZ in Asn and HEE, HEZ in Gln) 
have been individually assigned.

Each upper-bound constraint includes a brief comment(#)indicating the 
magnitude of the pseudo-atom correction factor which has been added 
to the value determined from the scheme outlined in Table I of
the article cited in the JRNL entry.  The basis for these values 
(i,g,m,r,q corresponding to 0.6,0.7,1.0,2.0,and 2.4 angstroms)is 
described in Wuethrich, et al.[J. Mol. Biol. 169: 949(1983)].

Additional comments are:
da-degenerate alpha proton resonances, NOE intensity divided by two;
db-degenerate beta proton resonances, NOE intensity divided by two;
dg-degenerate gamma proton resonances, NOE intensity divided by two;
dd-degenerate delta proton resonances, NOE intensity divided by two;
de-degenerate epsilon proton resonances, NOE intensity divided by two;
Me-methyl protons, NOE intensity divided by three;
BOTH-both NOESY crosspeaks between a given proton and the two protons
  of a single methylene could be established quantitatively, and both
  upper-bound constraints were assigned to the larger of the two 
  corresponding distances;
SS-CONSTR - disulfide constraint;
H-BOND - hydrogen-bond constraint.

The NOE-derived upperbound distance constraints were derived from 
three NOESY spectra: 65 ms mixing time in H2O (65 MS H2O); 100 ms
mixing time in D2O (100 MS H2O); and 250 ms mixing time in D2O 
(250 ms D2O).  Additional NOE-derived constraints which were derived 
from carefully baseline-corrected 65 ms mixing time data are indicated 
as 65 MS H2O LP (low plotting).



Table II.  Lower-bound Constraint List
	     Murine Epidermal Growth Factor
	      pH: 3.1, Temp: 28 deg

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