NMR Restraints Grid
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NMR Restraints Grid |
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Result table
| image | mrblock_id | pdb_id | cing | stage | program | type |
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28706 |
6gat   |
cing | 1-original | MR format | comment |
*HEADER COMPLEX (TRANSCRIPTION REGULATION/DNA) 07-NOV-97 6GAT *TITLE SOLUTION NMR STRUCTURE OF THE L22V MUTANT DNA BINDING *TITLE 2 DOMAIN OF AREA COMPLEXED TO A 13 BP DNA CONTAINING A TGATA *TITLE 3 SITE, REGULARIZED MEAN STRUCTURE *COMPND MOL_ID: 1; *COMPND 2 MOLECULE: AREA; *COMPND 3 CHAIN: A; *COMPND 4 FRAGMENT: DNA BINDING DOMAIN; *COMPND 5 ENGINEERED: YES; *COMPND 6 MUTATION: L22V; *COMPND 7 MOL_ID: 2; *COMPND 8 MOLECULE: DNA; *COMPND 9 CHAIN: B, C; *COMPND 10 ENGINEERED: YES *SOURCE MOL_ID: 1; *SOURCE 2 ORGANISM_SCIENTIFIC: ASPERGILLUS NIDULANS; *SOURCE 3 EXPRESSION_SYSTEM: ESCHERICHIA COLI; *SOURCE 4 MOL_ID: 2; *SOURCE 5 SYNTHETIC: YES *KEYWDS DNA BINDING PROTEIN, TRANSCRIPTION FACTOR, ZINC BINDING *KEYWDS 2 DOMAIN, COMPLEX (TRANSCRIPTION REGULATION/DNA) *EXPDTA NMR, REGULARIZED MEAN STRUCTURE *AUTHOR G.M.CLORE,M.STARICH,M.WIKSTROM,A.M.GRONENBORN *REVDAT 1 28-JAN-98 6GAT 0 ENTRY 6GATMR EXPERIMENTAL RESTRAINTS FOR 6GAT AND 7GAT TITLE SOLUTION STRUCTURE OF THE LEU22VAL MUTANT DNA BINDING DOMAIN OF TITLE AREA COMPLEXED TO A 13 BP DNA CONTAINING A TGATA SITE HEADER TRANSCRIPTION REGULATION EXPDTA NMR, REGULARIZED MEAN STRUCTURE AUTHOR G.M.CLORE, M. STARICH, M. WIKSTROM, A.M. GRONENBORN COMPND MOL_ID: 1; COMPND 2 MOLECULE: LEU22VAL MUTANT AREA DNA BINDING DOMAIN and DNA; COMPND 3 CHAIN: NULL SOURCE MOL_ID: 1; SOURCE 2 ORGANISM_SCIENTIFIC: ASPERGILUS NIDULANS SOURCE 3 EXPRESSION_SYSTEM: ESCHERICHIA COLI SOURCE 4 MOL_ID: 2; SOURCE 5 SYNTHETIC KEYWDS DNA BINDING PROTEIN, KEYWDS 2 TRANSCRIPTION FACTOR, ZINC BINDING DOMAIN JRNL AUTH M.R. STARICH, M. WIKSTROM, S SHUNACHER, H.N. ARST, JRNL AUTH 2 G.M.CLORE, A.M.GRONENBORN (1997) JRNL TITL SOLUTION STRUCTURE OF WILD TYPE JRNL TITL 2 AND MUTANT AREA PROTEIN-DNA COMPLEXES: DETERMINANTS OF AFFINITY AND JRNL TITL 3 SPECIFICITY IN GATA FACTORS. JRNL REF GENES & DEVELOPMENT IN PRESS REMARK 2 REMARK 2 RESOLUTION. NOT APPLICABLE. REMARK 3 REMARK 3 REFINEMENT. REMARK 3 PROGRAM : X-PLOR 3.1 REMARK 3 REMARK 3 OTHER REFINEMENT REMARKS: REMARK 3 THE STRUCTURES WERE CALCULATED USING THE SIMULATED REMARK 3 ANNEALING PROTOCOL OF NILGES ET AL. (1988) FEBS LETT. REMARK 3 229, 129 - 136 AND PROTEIN ENGINEERING 2, 27 - 38 USING THE PROGRAM REMARK 3 XPLOR MODIFIED TO INCORPORATE COUPLING CONSTANT RESTRAINTS (GARETT REMARK 3 ET AL. (1994) J. MAGN RESON. SERIES B 104, 99 - 103), CARBON REMARK 3 CHEMICAL SHIFT RETSRINATS (KUSZEWSKI ET AL. (1995) J. MAGN. RESON. REMARK 3 SERIES B 106, 92 - 96) RESTRAINTS, DIPOLAR COUPLING RESTRAINTS REMARK 3 (TJANDRA ET AL. (1997) NATURE STRUCT BIOL 4, 732-738) AND A REMARK 3 CONFORMATIONAL DATABASE POTENTIAL FOR PROTEINS AND NUCLEIC REMARK 3 ACIDS (KUSZWESKI ET AL. (1996) PROTEIN SCI 5, 1067 - 1080 REMARK 3 AND (1997) J. MAGN. RESON. 125, 171-177) REMARK 3
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