NMR Restraints Grid

Result table
 (Save to zip file containing files for each block)

image	mrblock_id	pdb_id	bmrb_id	cing	stage	program	type
	2647	1cdn	327	cing	1-original	MR format	comment

*HEADER   CALCIUM-BINDING PROTEIN                 04-AUG-95   1CDN    
*COMPND   MOL_ID: 1;                                                  
*COMPND  2 MOLECULE: CALBINDIN D9K;                                   
*COMPND  3 CHAIN: NULL;                                               
*COMPND  4 SYNONYM: INTESTINAL CALCIUM-BINDING PROTEIN, ICBP, ICABP,  
*COMPND  5 CABP9K, S100D;                                             
*COMPND  6 ENGINEERED: YES;                                           
*COMPND  7 MUTATION: INS(MET 1), P43G;                                
*COMPND  8 OTHER_DETAILS: BOVINE MINOR A FORM, CADMIUM-HALF-SATURATED,
*COMPND  9 CADMIUM ION IS BOUND IN C-TERMINAL SITE                    
*SOURCE   MOL_ID: 1;                                                  
*SOURCE  2 ORGANISM_SCIENTIFIC: BOS TAURUS;                           
*SOURCE  3 ORGANISM_COMMON: BOVINE;                                   
*SOURCE  4 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                       
*SOURCE  5 EXPRESSION_SYSTEM_PLASMID: PICB1;                          
*SOURCE  6 EXPRESSION_SYSTEM_GENE: ICABP                              
*KEYWDS   EF-HAND                                                     
*EXPDTA   NMR, 24 STRUCTURES                                          
*AUTHOR   M.AKKE,S.FORSEN,W.J.CHAZIN                                  
*REVDAT  1   14-NOV-95 1CDN    0                                      


HEADER   CALCIUM-BINDING PROTEIN 
COMPND   CALBINDIN D9K (BOVINE MINOR A FORM, CADMIUM-HALF-SATURATED, 
COMPND  2 CADMIUM ION IS BOUND IN THE C-TERMINAL SITE)
COMPND  3 (INTESTINAL CALCIUM BINDING PROTEIN, ICBP, ICABP, CABP9K, 
COMPND  4 S100D) MUTANT WITH N-TERMINAL MET, PRO 43 REPLACED BY GLY 
COMPND  5 (INS(MET 0),P43G) (NMR, 24 STRUCTURES)            
SOURCE   CALBINDIN D9K: BOVINE (BOS TAURUS)   
SOURCE  2 EXPRESSION SYSTEM: (ESCHERICHIA COLI) 
SOURCE  3 PLASMID: PICB1    
SOURCE  4 GENE: ICABP         
EXPDTA    NMR 
AUTHOR   M.AKKE,W.J.CHAZIN 
REVDAT 
REMARK   2 
REMARK   2 HYDROGEN ATOMS IN THIS RESTRAINT SET HAVE BEEN GIVEN 
REMARK   2 NAMES CONSISTENT WITH THE RECOMMENDATIONS OF THE IUPAC-IUB 
REMARK   2 COMMISSION ON BIOCHEMICAL NOMENCLATURE (SEE, E.G., J.MOL. 
REMARK   2 BIOL. 52, 1 (1970)), AND WHERE POSSIBLE, ARE CONSISTENT WITH 
REMARK   2 HYDROGEN ATOM NAMES IN THE CORRESPONDING PDB COORDINATE ENTRY 
REMARK   2 FOR (CD2+)1 CALBINDIN D9K.  
REMARK   2
REMARK   3
REMARK   3 (CD2+)1 CALBINDIN D9K NMR RESTRAINTS USED IN STRUCTURE
REMARK   3 DETERMINATION. COMPLETE LIST OF NOE AND DIHEDRAL ANGLE 
REMARK   3 RESTRAINTS.
REMARK   3
REMARK   3 DIHEDRAL ANGLE RESTRAINTS--                                       
REMARK   3   DIHEDRAL ANGLE RESTRAINTS ARE DERIVED FROM EXPERIMENTALLY 
REMARK   3   MEASURED 3JHNA, 3JAB AND 3JNHB COUPLING CONSTANTS IN 
REMARK   3   ADDITION TO NOES INVOLVING NH, CA, AND, CB PROTONS.  
REMARK   3   CHI-2 DIHEDRAL ANGE RESTRAINTS WERE INFERRED BY COMPARISON
REMARK   3   OF THE EXPERIMENTAL AND THEORETICAL RELATIVE NOE 
REMARK   3   INTENSITIES FOR THE INTRA-RESIDUE HB-HD CROSS-PEAKS, AS
REMARK   3   DESCRIBED IN THE PAPER CITED IN THE JRNL RECORD OF THE
REMARK   3   COORDINATE FILE.
REMARK   3   IN TOTAL THERE ARE 40 PHI, 27 CHI1 AND 4 CHI2 CONSTRAINTS.
REMARK   3
REMARK   3      TABLE OF DIHEDRAL ANGLE RESTRAINT BOUNDS
REMARK   3   -------------------------------------------------------

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